Difference between revisions of "Part:BBa K2097000"
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<partinfo>BBa_K2097000 short</partinfo>
 
<partinfo>BBa_K2097000 short</partinfo>
 
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<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but <i>E. coli</i> has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site.  Used <a href="https://parts.igem.org/Part:BBa_K2097002">BBa_K2097002</a> as the control series.</p></html>
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<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor for the downstream gene, YGCP in this case. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but <i>E. coli</i> has a homolog of these proteins so only the appropriate BioBrick prefix/suffix and CpxR binding site were required.  Used <a href="https://parts.igem.org/Part:BBa_K2097002">BBa_K2097002</a> as the control series.</p></html>
  
 
<h3>Usage and Biology</h3><p></p>
 
<h3>Usage and Biology</h3><p></p>

Revision as of 17:54, 20 October 2016


CpxR binding site attached to a yellow-green color protein (YGCP) acts as a neutral pH indicator.

CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor for the downstream gene, YGCP in this case. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so only the appropriate BioBrick prefix/suffix and CpxR binding site were required. Used BBa_K2097002 as the control series.

Usage and Biology

T--Austin_UTexas--pH_Dependent_Promoter.jpeg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]