Difference between revisions of "Part:BBa K2082002"
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This part can be used in combination with <a href="https://parts.igem.org/Part:BBa_K2082002">BBa_K2082002</a> to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.
  
 
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Revision as of 17:26, 20 October 2016


Split CFP Bimolecular fluorescence complementation (BiFC) with anti-GFP-Nanobody

Anderson promoter, RBS, bindin anti-GFP-Nanobody CDS and Split fluorescent protein (CFP) CDS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 215
    Illegal AgeI site found at 467
    Illegal AgeI site found at 590
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a RBS (BBa_B0034)
  • a anti-GFP-Nanobody CDS
  • a N-terminal split-CFP CDS

This part can be used in combination with <a href="https://parts.igem.org/Part:BBa_K2082002">BBa_K2082002</a> to evaluate the binding properties of the anti-GFP-Nanobody. The attachment of GFP and the anti-GFP-Nanobody leads to the completion of the split CFP domains. and so to a blue fluorescence.