Difference between revisions of "Part:BBa K1900002"

 
 
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<partinfo>BBa_K1900002 short</partinfo>
 
<partinfo>BBa_K1900002 short</partinfo>
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Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the E. coli tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 16:59, 20 October 2016


pBAD+strong RBS+E. coli tolC signal sequence+E. coli tolC Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the E. coli tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
    Illegal NheI site found at 1615
    Illegal NheI site found at 1636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1555
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1110