Difference between revisions of "Part:BBa K1900001:Design"

 
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===Design Notes===
 
===Design Notes===
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BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Serratia marcescens TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.
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===Source===
 
===Source===

Revision as of 16:56, 20 October 2016


pBAD+strong RBS+E. coli tolC signal sequence+S. marcescens tolC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
    Illegal NheI site found at 1663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1582
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 1126
    Illegal NgoMIV site found at 1413
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K206000 was chosen for its ability to be controlled with a common inducer (arabinose) as well as its high rate of transcription. BBa_B0034 was chosen for its high translation efficiency. The main consideration when designing the signaling sequence and Serratia marcescens TolC gene was to use silent mutations alter any RFC10 incompatible DNA sections to be biobrick compatible.

Source

This part

References