Difference between revisions of "Part:BBa K1900001"

Revision as of 16:55, 20 October 2016

pBAD+strong RBS+E. coli tolC signal sequence+S. marcescens tolC

Combination of part BBa_K1406000 (BBa_K206000 + BBa_B0034) the E. coli tolC signaling sequence and the Serratia marcescens tolC gene. Transcription is controlled by the arabinose-induced pBAD promoter (BBa_K206000). A strong E. coli RBS (BBa_B0034) controls translation. The signaling sequence is a cleavable N-terminal signaling sequence from E. coli's tolC gene. This is required for trimerization of the TolC monomers as well as insertion into the outer membrane; it forms a chimeric polypeptide with the tolC gene from Serratia marcescens. TolC trimers form a outer-membrane alpha/beta barrel pore can interface with a variety of molecule pumps to form a transperiplasmic pump.