Difference between revisions of "Part:BBa K2082001"
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For creation of a unique Nanobody library you can use our framework, containing the constant Nanobody segments. Amplifying <a href=https://parts.igem.org/wiki/index.php?title=Part:BBa_K2082001>BBa_K2082001</a> with primers <a href="http://2016.igem.org/Team:Bielefeld-CeBiTec/Experiments/Primers">NB-bb-fw</a> and <a href="http://2016.igem.org/Team:Bielefeld-CeBiTec/Experiments/Primers">NB-bb-rev</a> results to a backbone, to insert a double stranded and overlapping variable insert by Gibson assembly, to built a unique Nanobody library.
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For creation of a unique Nanobody library you can use our framework, containing the constant Nanobody segments. Amplifying <a href=https://parts.igem.org/wiki/index.php?title=Part:BBa_K2082001>BBa_K2082001</a> with primers <a href="http://2016.igem.org/Team:Bielefeld-CeBiTec/Experiments/Primers">NB-bb-fw</a> and <a href="http://2016.igem.org/Team:Bielefeld-CeBiTec/Experiments/Primers">NB-bb-rev</a> results to a backbone, to insert a double stranded and overlapping variable insert by Gibson assembly.
 
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Revision as of 16:29, 20 October 2016


Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)

Pand-RBS-rpoZ CDS-cMyc linker-Nanobody constant regions-Terminator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 166
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This device consists of:

  • an Anderson promoter (BBa_J23106)
  • a RBS (BBa_B0034)
  • a rpoZ CDS (encoding RNA polymerase omega subunit)
  • a c-Myc linker
  • Nanobody CDS constant regions
For creation of a unique Nanobody library you can use our framework, containing the constant Nanobody segments. Amplifying BBa_K2082001 with primers NB-bb-fw and NB-bb-rev results to a backbone, to insert a double stranded and overlapping variable insert by Gibson assembly.