Difference between revisions of "Part:BBa K1983006:Design"
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Original suffix: TACTAGTAGCGGCCGCTGCAG | Original suffix: TACTAGTAGCGGCCGCTGCAG | ||
Part suffix: GACTAGTAGCGGCCGCTGCAG | Part suffix: GACTAGTAGCGGCCGCTGCAG | ||
+ | |||
+ | ===Gp45M37 mutation list=== | ||
+ | |||
+ | L220F; L23F; Y172F; I107F; M22F; M187F; Y55F; M194F; L197F; W199F; L167F; Y194F; Y39F; Y216F; M184F; M30F; L10F; I48F; L64F; L127F; L123F; L61F; I54F; I147F; I140F; L160F; I181F; L11F; I83F; I73F; L126F; L57F; I149F; I81F; I17F; I29F; I44F; | ||
===Source=== | ===Source=== |
Revision as of 15:31, 20 October 2016
gp45 phenylalanine mutant M37 with C-terminal 6XHis-Tag
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 688
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed including NdeI restriction site before the start codon (ATG) for efficient cloning into expression vectors, XhoI site for cutting between the protein and 6XHis-Tag.
Note: Due to technical issues, the part's suffix in the backbone is changed by one FIRST letter (T to G). However, it does not remove or add any other restriction sites and does not change the function of the suffix. The purpose of this note is to alert false negative results during sequencing if the part is used in the future.
Original suffix: TACTAGTAGCGGCCGCTGCAG Part suffix: GACTAGTAGCGGCCGCTGCAG
Gp45M37 mutation list
L220F; L23F; Y172F; I107F; M22F; M187F; Y55F; M194F; L197F; W199F; L167F; Y194F; Y39F; Y216F; M184F; M30F; L10F; I48F; L64F; L127F; L123F; L61F; I54F; I147F; I140F; L160F; I181F; L11F; I83F; I73F; L126F; L57F; I149F; I81F; I17F; I29F; I44F;
Source
T4 phage