Difference between revisions of "Part:BBa K2014004"
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<partinfo>BBa_K2014004 short</partinfo> | <partinfo>BBa_K2014004 short</partinfo> | ||
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+ | The promoter pxylS-M5'UTR (with a lab name: XylS-UTR ), tested in a biobrick pxylS-M5'UTR->sfGFP (BBa_K2014004) is a modified xylose-induced promoter/5’UTR controlling sfGFP protein expression. To make it we exchanged the 5’UTR sequence of xylA-proD5'UTR in a biobrick <b>(BBa_K1741009)</b> to a synthetic, unstructured M5’UTR (picture B) derived from the Mel2 promoter <b>(BBa_K1741004)</b>, which we provided to iGEM Registry in 2015. | ||
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+ | If you are interested in the pathway of our modifications please click here-> <a href="https://static.igem.org/mediawiki/parts/c/c4/BBa_K2014004.pdf">MODIFICATIONS OF XYLOSE INDUCED PROMOTERS</a> | ||
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Long Description goes here | Long Description goes here |
Revision as of 12:30, 20 October 2016
pxylS-M5'UTR->sfGFP
The promoter pxylS-M5'UTR (with a lab name: XylS-UTR ), tested in a biobrick pxylS-M5'UTR->sfGFP (BBa_K2014004) is a modified xylose-induced promoter/5’UTR controlling sfGFP protein expression. To make it we exchanged the 5’UTR sequence of xylA-proD5'UTR in a biobrick (BBa_K1741009) to a synthetic, unstructured M5’UTR (picture B) derived from the Mel2 promoter (BBa_K1741004), which we provided to iGEM Registry in 2015.
If you are interested in the pathway of our modifications please click here-> <a href="https://static.igem.org/mediawiki/parts/c/c4/BBa_K2014004.pdf">MODIFICATIONS OF XYLOSE INDUCED PROMOTERS</a>
Long Description goes here
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 162