Difference between revisions of "Part:BBa K1927003"

Revision as of 11:42, 20 October 2016

Beta - lactamase AmpR

This part is an ampicillin resistant gene with the J04500 inducible promotor.

Usage and Biology

Applications of BBa_K1927003

File:Https://static.igem.org/mediawiki/parts/8/88/Pcr003.jpg

alt text

Figure3: Gel analysis of colony PCR with BBa_K1927003. The insert was inserted into the shipping vector pSB1C3 by using 2 different approaches; 3A assembly and a single restriction digest followed by ligation. Lane 1 and 10 are DNA ladder (GeneRuler 1 kb DNA ladder, Thermo Fisher Scientific). Lane 2 and 11 are positive control (validated part BBa_K1927000). Lane 3-7 are different colonies from 3A assembly. Lane 8-9 and 12-14 are restriction digest and ligation approach. Lane 4, 6-8, 12-13 shows the appropriate band at approx. 1000bp.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 942

@@ Line 4: / Line 4: @@
 This part is an ampicillin resistant gene with the J04500 inducible promotor.
+===Usage and Biology===
 ===Applications of BBa_K1927003===
+[[File:https://static.igem.org/mediawiki/parts/8/88/Pcr003.jpg|200px|thumb|left|alt text]]
+Figure3: Gel analysis of colony PCR with BBa_K1927003. The insert was inserted into the shipping vector pSB1C3 by using 2 different approaches; 3A assembly and a single restriction digest followed by ligation. Lane 1 and 10 are DNA ladder (GeneRuler 1 kb DNA ladder, Thermo Fisher Scientific). Lane 2 and 11 are positive control (validated part BBa_K1927000). Lane 3-7 are different colonies from 3A assembly.  Lane 8-9 and 12-14 are restriction digest and ligation approach. Lane 4, 6-8, 12-13 shows the appropriate band at approx. 1000bp.
 <!-- Add more about the biology of this part here
-===Usage and Biology===