Difference between revisions of "Part:BBa K1927003"

 
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===Usage and Biology===
 
===Usage and Biology===
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===Applications of BBa_K1927003===
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[[File:https://static.igem.org/mediawiki/parts/8/88/Pcr003.jpg|200px|thumb|left|Figure3: Gel analysis of colony PCR with BBa_K1927003. The insert was inserted into the shipping vector pSB1C3 by using 2 different approaches; 3A assembly and a single restriction digest followed by ligation. Lane 1 and 10 are DNA ladder (GeneRuler 1 kb DNA ladder, Thermo Fisher Scientific). Lane 2 and 11 are positive control (validated part BBa_K1927000). Lane 3-7 are different colonies from 3A assembly.  Lane 8-9 and 12-14 are restriction digest and ligation approach. Lane 4, 6-8, 12-13 shows the appropriate band at approx. 1000bp. ]]
  
 
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Revision as of 11:40, 20 October 2016


Beta - lactamase AmpR

This part is an ampicillin resistant gene with the J04500 inducible promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 942