Difference between revisions of "Part:BBa K2120420"
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<partinfo>BBa_K2120420 short</partinfo> | <partinfo>BBa_K2120420 short</partinfo> | ||
| + | This is one of our application circuit. | ||
| + | Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks. | ||
| + | |||
| + | We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system. | ||
| + | |||
| + | Go our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2016&group=BIT-China Parts Registry] to learn more. | ||
Revision as of 08:28, 20 October 2016
B0015+tetR+B0034+J23106+ptet+B0034+mRFP+B0015
This is one of our application circuit.
Our application circuit circuit consists of two parts:the inhibitor device and killer device ,which have already been made into basic parts of biobricks.
We choose four different promoters whose reported strengths are respectively low, medium,strong and the strongest from constitutive promoter family to express the inhibitor tetR and cI ,and then we construct three plasmid with different copy number containing this circuit, in order to find out the proper promoter which meets the threshold that trigger the killer system.
Go our Parts Registry to learn more.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 851
Illegal NheI site found at 874 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1528
Illegal AgeI site found at 1640 - 1000COMPATIBLE WITH RFC[1000]