Difference between revisions of "Part:BBa B0015:Experience"

Revision as of 21:08, 23 August 2007

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_B0015

PCR Problems: Using primers VR and VF2 to PCR B0010 results in excess bands. VR can anneal to B0010, resulting in shorter bands than expected. A full description of the problem is available here.
We used this piece to terminate many of our Berkeley iGEM 2005 Parts. It appeared to work quite well. [Smelissali 6/7/06]
There have been reports from the Davidson iGEM team that the plasmid from the iGEM 2006 Repository for this part did NOT contain this biobrick. If you are experiencing this or a similar concern, please contact either myself or [http://parts.mit.edu/wiki/index.php/User:Meaganl Meagan Lizarazo] [--Smelissali 14:26, 2 August 2006 (EDT)]

User Reviews

UNIQc9a589a92c307c8e-partinfo-00000000-QINU

••••

Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQc9a589a92c307c8e-partinfo-00000002-QINU

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 ===Applications of BBa_B0015===
+*'''PCR Problems:''' Using primers VR and VF2 to PCR B0010 results in excess bands. VR can anneal to B0010, resulting in shorter bands than expected. A full description of the problem is available [[Problems with PCR using VR/VF2|here]].
 *We used this piece to terminate many of our [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM&group=iGEM_Berkeley| Berkeley iGEM 2005 Parts].  It appeared to work quite well. [Smelissali 6/7/06]
 *There have been reports from the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=iGEM2006_Davidson Davidson iGEM team] that the plasmid from the [https://parts.igem.org/cgi/assembly/libraries.cgi?id=6 iGEM 2006 Repository] for this part did NOT contain this biobrick.  If you are experiencing this or a similar concern, please contact either [[User:Smelissali|myself]] or [http://parts.mit.edu/wiki/index.php/User:Meaganl Meagan Lizarazo] [--[[User:Smelissali|Smelissali]] 14:26, 2 August 2006 (EDT)]