Difference between revisions of "Part:BBa K2066039:Design"

 
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===Design Notes===
 
===Design Notes===
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to be completed
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This part is designed for Gibson Assembly from the 57S plasmid from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper, with a UNS backbone to create K2066038. Note that this part has no double terminator, as the original 57S plasmid did not have one after the mCherry + NRII coding regions.
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===Source===
 
===Source===

Revision as of 03:15, 20 October 2016


Sigma54 Enhancer 57S with DT, UNS Standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 111
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for Gibson Assembly from the 57S plasmid from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper, with a UNS backbone to create K2066038. Note that this part has no double terminator, as the original 57S plasmid did not have one after the mCherry + NRII coding regions.

Source

to be completed

References