Difference between revisions of "Part:BBa K1898400"

Revision as of 03:00, 20 October 2016

CRYAB, crystallin alpha B

This gene codes for CRAYB, also known as crystallin alpha B, a crystallin protein exists in the lens and also other tissues and organs.

Usage and Biology

Alpha crystallin is the most abundant protein in the lens. Out of the two types of alpha crystallin proteins, alpha B is less restricted to the lens and has a 1:3 ratio to alpha A [1]. It has been found that crystallin alpha B affects lens development and has chaperone activity to maintain normal protein interaction[2].

In our project, we made CRYAB because we wanted to monitor crystallin protein's response to oxidative stress and to test our prevention and treatment for cataracts.

[1]Genes and mapped phenotypes. (n.d.). from https://www.ncbi.nlm.nih.gov/gene/1410

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 26
Illegal BamHI site found at 368
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 453
Illegal SapI.rc site found at 313

Gel Picture

We designed primers to move CRYAB cDNA into iGEM BioBrick. PCR was set up and the gel picture is shown below. The expected band size is ~1kb, and the bands at the expected size are boxed in red.

Sequencing

We sent CRYAB out to sequencing after it's cloned into iGEM backbone. The sequencing file is annotated as follows:

four restriction sites are highlighted in red
CRYAB is highlighted in orange

This confirms that the sequence is correct (with no stop codon) and is moved into the BioBrick.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1898400 short</partinfo>
-This gene codes for cryab, also known as crystallin alpha B, a crystallin protein exists in the lens and also other tissues and organs.
+This gene codes for CRAYB, also known as crystallin alpha B, a crystallin protein exists in the lens and also other tissues and organs.
 ===Usage and Biology===
 Alpha crystallin is the most abundant protein in the lens. Out of the two types of alpha crystallin proteins, alpha B is less restricted to the lens and has a 1:3 ratio to alpha A [1]. It has been found that crystallin alpha B affects lens development and has chaperone activity to maintain normal protein interaction[2].
-In our project, we made CRYAA because we wanted to monitor crystallin protein's response to oxidative stress and to test our prevention and treatment for cataracts.
+In our project, we made CRYAB because we wanted to monitor crystallin protein's response to oxidative stress and to test our prevention and treatment for cataracts.
 [1]Genes and mapped phenotypes. (n.d.). from https://www.ncbi.nlm.nih.gov/gene/1410