Difference between revisions of "Part:BBa K2150115"

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This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.  
 
This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.  
  
See: [https://parts.igem.org/Part:BBa_K2150111 BBa_K2150111]
+
See: [https://parts.igem.org/Part:BBa_K2150117 BBa_K2150117]
  
 +
We also characterized its corresponding toxin, ParE, see:
 +
[https://parts.igem.org/Part:BBa_K2150111 BBa_K2150111]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 02:54, 20 October 2016


6249 antitoxin tet

This antitoxin belongs to ParD family. It can effectively neutralize the toxin ParE. We characterized this by measuring the growth curve co-expressing toxin and antitoxin.

This protein is induced by aTc. When studying the interaction between antitoxin and toxin, antitoxin is induced by IPTG.

See: BBa_K2150117

We also characterized its corresponding toxin, ParE, see: BBa_K2150111 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]