Difference between revisions of "Part:BBa K2100007:Experience"
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===Applications of BBa_K2100007=== | ===Applications of BBa_K2100007=== | ||
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| + | Our team used hEF1a-tagBFP as a transfection marker for some of our experiments. This particular experiment characterizing the EGSH promoter measured blue fluorescent output from hEF1a-tagBFP to analyze how many copy numbers of plasmids the cells uptook. The graph shows an increase in blue fluorescence corresponds with an increase in red fluorescence at the basal level (uninduced without the coactivator), indicating that when more hEF1a-BFP plasmids are uptaken, so is the plasmid of interest, in this case pEGSH-mKate. | ||
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| + | https://static.igem.org/mediawiki/parts/1/17/T--MIT--EGSH.png | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:43, 20 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2100007
Our team used hEF1a-tagBFP as a transfection marker for some of our experiments. This particular experiment characterizing the EGSH promoter measured blue fluorescent output from hEF1a-tagBFP to analyze how many copy numbers of plasmids the cells uptook. The graph shows an increase in blue fluorescence corresponds with an increase in red fluorescence at the basal level (uninduced without the coactivator), indicating that when more hEF1a-BFP plasmids are uptaken, so is the plasmid of interest, in this case pEGSH-mKate.
User Reviews
UNIQ56b009e3d114c067-partinfo-00000000-QINU UNIQ56b009e3d114c067-partinfo-00000001-QINU