Difference between revisions of "Part:BBa K1886007"

 
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<partinfo>BBa_K1886007 parameters</partinfo>
 
<partinfo>BBa_K1886007 parameters</partinfo>
 
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<h2>'''Characterization'''</h2>
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<h3> BACKGROUND </h3>
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<h4>Overview</h4>
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Registered Parts in our wiki is the subunit of the entire AND Gate system, each of which has no function. So I will introduce our logical part of our project after the combination of all the subunits in the page of part [https://parts.igem.org/Part:BBa_K1886016 BBa_K1886016].
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<br><br>
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<h3> Results </h3>
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<h4>Gel electrophoretic analysis</h4>

Revision as of 02:36, 20 October 2016


AND GATE-input

This is the input part of AND GATE. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by L-arabinose, pBAD promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 174


Characterization

BACKGROUND

Overview

Registered Parts in our wiki is the subunit of the entire AND Gate system, each of which has no function. So I will introduce our logical part of our project after the combination of all the subunits in the page of part BBa_K1886016.

Results

Gel electrophoretic analysis