Difference between revisions of "Part:BBa K1894000"

Revision as of 02:09, 20 October 2016

Modified GvpA1 coding region

The gene GvpA1 can express GvpA protein, which acts as the main structural protein of gas vesicle in Microcystis aeruginosa. We mutate GvpA1 gene in order to limit the level of GvpA1 expression and change the conformation of gas vesicle protein---- a possible strategy to disrupt the distribution of microcysis in water and control algae bloom.By changing two base pairs, we mutate a serine to an alanine (hydrophilic amino acid to hydrophobic amino acid) and an alanine to a glycin.

Usage and Biology

Gas vesicle protein, which widely exists in all forms of planktonic cyanobacteria, provides cells with buoyancy by changing the density of cells and regulates their vertical distributions in natural waters, enabling them to achieve ideal vertical position in water for growth and subsequent niche colonization. GvpA protein forms the skeleton of gas vesicle and acts as the main structural protein. Therefore, we plans to mutate GvpA1 gene in order to limit the level of GvpA1 expression and change the conformation of gas vesicle protein---- a possible strategy to disrupt the distribution of microcysis in water and control algae bloom. By changing two base pairs, we mutate a serine to an alanine (hydrophilic amino acid to hydrophobic amino acid) and an alanine to a glycin.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1894000 short</partinfo>
-The gene GvpA1 can express GvpA protein, which acts as the main structural protein of gas vesicle in Microcystis aeruginosa.Gas vesicle protein, which widely exists in all forms of planktonic cyanobacteria, provides cells with buoyancy by changing the density of cells and regulates their vertical distributions in natural waters, enabling them to achieve ideal vertical position in water for growth and subsequent niche colonization. Since floating and gathering together are the last and most critical stage in the formation of algae bloom,we mutate GvpA1 gene in order to limit the level of GvpA1 expression and change the conformation of gas vesicle protein---- a possible strategy to disrupt the distribution of microcysis in water and control algae bloom.By changing two base pairs, we mutate a serine to an alanine (hydrophilic amino acid to hydrophobic amino acid) and an alanine to a glycin.
+The gene GvpA1 can express GvpA protein, which acts as the main structural protein of gas vesicle in Microcystis aeruginosa. We mutate GvpA1 gene in order to limit the level of GvpA1 expression and change the conformation of gas vesicle protein---- a possible strategy to disrupt the distribution of microcysis in water and control algae bloom.By changing two base pairs, we mutate a serine to an alanine (hydrophilic amino acid to hydrophobic amino acid) and an alanine to a glycin.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Gas vesicle protein, which widely exists in all forms of planktonic cyanobacteria, provides cells with buoyancy by changing the density of cells and regulates their vertical distributions in natural waters, enabling them to achieve ideal vertical position in water for growth and subsequent niche colonization.
+GvpA protein forms the skeleton of gas vesicle and acts as the main structural protein. Therefore, we plans to mutate GvpA1 gene in order to limit the level of GvpA1 expression and change the conformation of gas vesicle protein---- a possible strategy to disrupt the distribution of microcysis in water and control algae bloom. By changing two base pairs, we mutate a serine to an alanine (hydrophilic amino acid to hydrophobic amino acid) and an alanine to a glycin.
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