Difference between revisions of "Part:BBa K2055000"
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DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein. | DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein. | ||
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| + | In C.Violaceum, this part expressing the RFP BBa_K51603, did not presented an inducible activity in the e-waste copper and gold compositions that Tec-Monterrey 2016 worked with. Nevertheless, a basal activity for the fluorescence was found in the following graph. | ||
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| + | [Image:https://static.igem.org/mediawiki/parts/f/fc/T--Tec-Monterrey--violaceumpgols.jpg] | ||
Latest revision as of 01:45, 20 October 2016
Gold/copper inducible regulator
DNA construct constituted by the pgolS promoter and the coding sequence of CueR. As the pgolS promoter has a basal transcription level, some CueR would be constantly expressed. CueR binds either copper or gold, though it is more selective for copper. CueR is a transcription factor that stimulates the transcription at the pgolS promoter when bound to copper/gold. Thus, this construct increases its expression in the presence of gold or copper through the production of a copper/gold sensitive protein.
In C.Violaceum, this part expressing the RFP BBa_K51603, did not presented an inducible activity in the e-waste copper and gold compositions that Tec-Monterrey 2016 worked with. Nevertheless, a basal activity for the fluorescence was found in the following graph.
[Image:
]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 93
Illegal AgeI site found at 1086
Illegal AgeI site found at 1198 - 1000COMPATIBLE WITH RFC[1000]