Difference between revisions of "Part:BBa K2066059:Design"

 
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<partinfo>BBa_K206659 short</partinfo>
  
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<partinfo>BBa_K206659 SequenceAndFeatures</partinfo>
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===Design Notes===
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This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”).
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===Source===
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This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.

Revision as of 00:59, 20 October 2016

No part name specified with partinfo tag.

No part name specified with partinfo tag.


Design Notes

This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”).


Source

This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.