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[[File:Pompc_CI_p-lamda_sfGFP.tiff|800px|thumb|left|Fig.1 Fig.1 Gel electrophoretic analyses of PCR products ]]
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[[File:Pompc_CI_p-lamda_sfGFP.tif|800px|thumb|left|Fig.1 Fig.1 Gel electrophoretic analyses of PCR products ]]
  
 
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Revision as of 00:12, 20 October 2016


Pompc_CI_p-lamda_sfGFP

We inserted a CI protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1141


Characterization

Principle

Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system. Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.

Gel electrophoretic analysis


File:Pompc CI p-lamda sfGFP.tif