Difference between revisions of "Part:BBa K1645999"
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<partinfo>BBa_K1645999 short</partinfo>
 
<partinfo>BBa_K1645999 short</partinfo>
  
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This part is a composite of the full GFP cassette (taken from registry part BBa_I20260) and the full RFP cassette (taken from registry part BBa_J04450). The GFP is under the constitutive J23101 promoter. The RFP is under the lacI (R0010) promoter, which is constitutive in DH5-alpha because lacI is not expressed. RFP expression is inhibited if lacI is present in the cell or if IPTG is not present. This part allowed us to visualise colour swaps caused by different SgRNA targeting the promoter region of the two colours colours.
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=Dual Colour Reporter Plasmid=
  
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<!-- Add more about the biology of this part here
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This part is a composite of an RFP gene and a GFP gene from the previously made BioBricks: BBa_I20260 and BBa_J04450, respectively.
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===Usage and Biology===
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It can be used in CRISPRi experiments to demonstrate the effectiveness of sgRNA-Cas9 targeting. It can also be used for other gene expression suppression systems to provide quantitative feedback and data.
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It has been characterized using flow cytometry to demonstrate the expression of both RFP and GFP proteins.
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Further characterization of this part being used in a CRISPRi system can be found with the BBa_K1645998 BioBrick.
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==Characterization==
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TEst
  
 
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Revision as of 00:08, 20 October 2016

Dual Color Plasmid (RFP and GFP)

Dual Colour Reporter Plasmid

This part is a composite of an RFP gene and a GFP gene from the previously made BioBricks: BBa_I20260 and BBa_J04450, respectively.

It can be used in CRISPRi experiments to demonstrate the effectiveness of sgRNA-Cas9 targeting. It can also be used for other gene expression suppression systems to provide quantitative feedback and data.

It has been characterized using flow cytometry to demonstrate the expression of both RFP and GFP proteins.

Further characterization of this part being used in a CRISPRi system can be found with the BBa_K1645998 BioBrick.

Characterization

TEst

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1708
    Illegal AgeI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 706