Difference between revisions of "Part:BBa K2066538"
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<partinfo>BBa_K2066538 short</partinfo> | <partinfo>BBa_K2066538 short</partinfo> | ||
− | NRII mutant lacking phosphatase activity under the control of the Ntr operon promoter. | + | NRII mutant lacking phosphatase activity under the control of the Ntr operon promoter. This is the NRII2302 coding sequence from the pACT Tet plasmid from Amit et. al. 2011. The sequence is from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). |
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Latest revision as of 00:06, 20 October 2016
Synthetic Enhancer Project: NRII2302 Mutant Coding
NRII mutant lacking phosphatase activity under the control of the Ntr operon promoter. This is the NRII2302 coding sequence from the pACT Tet plasmid from Amit et. al. 2011. The sequence is from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 917
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]