Difference between revisions of "Part:BBa K2066126"

 
 
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This is the upstream region of NRII2302 mutant coding region in the pACT Tet plasmid from Amit et. al.  It contains the promoter that drives the expression of NRII2302, a mutated protein that only has kinase activity that allows it to phosphorylate the NRI protein to let it activate and bind to the enhancer region of the synthetic enhancer circuit.
  
 
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Latest revision as of 23:56, 19 October 2016


Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter

This is the upstream region of NRII2302 mutant coding region in the pACT Tet plasmid from Amit et. al. It contains the promoter that drives the expression of NRII2302, a mutated protein that only has kinase activity that allows it to phosphorylate the NRI protein to let it activate and bind to the enhancer region of the synthetic enhancer circuit.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]