Difference between revisions of "Part:BBa K2150017"

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<partinfo>BBa_K2150017 short</partinfo>
 
<partinfo>BBa_K2150017 short</partinfo>
  
T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator.  
+
T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP, BBa_K2150013) with double terminator.  
  
  

Revision as of 23:55, 19 October 2016


pT7 TetX-GFP(fusion protein)

T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP, BBa_K2150013) with double terminator.


Usage and Biology

This part is designed to produce a high level of the degrading enzyme in the presence of T7 RNA polymerase(see BBa_K21500028).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 608
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1905