Difference between revisions of "Part:BBa K1886018"
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<h2>'''Characterization'''</h2>
 
<h2>'''Characterization'''</h2>
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<h3> BACKGROUND </h3>
 
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<h4>Overview</h4>
 
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Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system.
 
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<br>
 
 
<h4>Principle</h4>
 
<h4>Principle</h4>
 +
Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system.
 
Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.
 
Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.
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<br>
 
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<h3>RESULTS</h3>
 
 
<br>
 
<br>
 
<h4>Gel electrophoretic analysis</h4>
 
<h4>Gel electrophoretic analysis</h4>

Revision as of 23:51, 19 October 2016


Pompc_CI_p-lamda_sfGFP

We inserted a CI protein-terminator-Pλ between Pompc and sfGFP to change the red-TCS from being activated by darkness and inhibited by red light into being activated by red light but inhibited by darkness.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1141


Characterization

Principle

Considering that PompC is a red light inhibited promoter, we designed a NOT Gate to realize our purpose of a light-activating system. Pλ is a promoter which can be inhibited by protein CI. We use this part combined with CI to realize the main function of regulating the whole system.

Gel electrophoretic analysis