Difference between revisions of "Part:BBa K2066031"
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<partinfo>BBa_K2066031 short</partinfo>
 
<partinfo>BBa_K2066031 short</partinfo>
  
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pTAC Ribozyme Characterization Part – cIGFP only; no RiboJ
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This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship without RiboJ.  
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This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_030 (pTAC Ribozyme Chracterization Part – GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:37, 19 October 2016

pTAC cI-GFP

This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship without RiboJ.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 852