Difference between revisions of "Part:BBa K2066531"
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<partinfo>BBa_K2066531 short</partinfo> | <partinfo>BBa_K2066531 short</partinfo> | ||
| − | . | + | 52S binding cassette has three TetO binding sites. Based on the amount of aTc in the system, which binds and inactivates TetR repressor, the amount of Tetr bound to these two TetO varies and thus flexibility of the DNA is also changing and ultimately the looping between the enhancer and promoter and output levels. These three TetO sites on 52S allows for four discrete states: unrepressed, two intermediate steps, completely repressed. This part is from a plasmid gifted by Amit et. al 2011. |
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| + | Source: Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). | ||
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Latest revision as of 23:30, 19 October 2016
Synthetic Enhancer Project: 3X TetO Binding Cassette(52S)
52S binding cassette has three TetO binding sites. Based on the amount of aTc in the system, which binds and inactivates TetR repressor, the amount of Tetr bound to these two TetO varies and thus flexibility of the DNA is also changing and ultimately the looping between the enhancer and promoter and output levels. These three TetO sites on 52S allows for four discrete states: unrepressed, two intermediate steps, completely repressed. This part is from a plasmid gifted by Amit et. al 2011.
Source: Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 24
Illegal NheI site found at 119 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 84
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]