Difference between revisions of "Part:BBa K1909011"

Latest revision as of 22:51, 19 October 2016

Medium Anderson / Taz

Ba_K1909011 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor tar-envZ, part BBa_C0082, which is a combination of the chemotaxis receptor Tar capable of sensing aspartate, and the cytosolic domain of osmosensor kinase EnvZ.

Functional Parameters

This combination has a higher expression as BBa_K1909012 and a lower expression as BBa_K1909010. Testing the combination showed that the cells grew slowly (for miniprep culture more than 24 hours). We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 173

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1909011 short</partinfo>
-Coming Soon
+Ba_K1909011 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor tar-envZ, part BBa_C0082, which is a combination of the chemotaxis receptor Tar capable of sensing aspartate, and the cytosolic domain of osmosensor kinase EnvZ.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Functional Parameters===
+This combination has a higher expression as BBa_K1909012 and a lower expression as BBa_K1909010.
+Testing the combination showed that the cells grew slowly (for miniprep culture more than 24 hours).
+We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>