Difference between revisions of "Part:BBa K1909008"
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<partinfo>BBa_K1909008 short</partinfo> | <partinfo>BBa_K1909008 short</partinfo> | ||
− | + | BBa_K1909008 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP). | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608006 and BBa_C0082 is part BBa_K1909011. | ||
+ | This combination has a higher expression as BBa_K1909009 and a lower expression as BBa_K1909007. | ||
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+ | ===Functional Parameters=== | ||
+ | |||
+ | Testing the combination showed that cells grew slowly (for miniprep culture more than 24 hours). | ||
+ | We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor. | ||
+ | |||
+ | |||
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Revision as of 22:35, 19 October 2016
Medium Anderson / mTaz
BBa_K1909008 is a construct of medium Anderson promoter BBa_K608006, a strong ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).
Usage and Biology
The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608006 and BBa_C0082 is part BBa_K1909011. This combination has a higher expression as BBa_K1909009 and a lower expression as BBa_K1909007.
Functional Parameters
Testing the combination showed that cells grew slowly (for miniprep culture more than 24 hours). We assume that this combination (medium promoter and strong RBS) induces a high expression of the transmembrane receptor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 173