Difference between revisions of "Part:BBa K1886013"

Latest revision as of 22:32, 19 October 2016

AND GATE-input_DPD+ red light

The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations(T7ptag) . The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CI protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CI protein(for more information, see the part"PompC-NOT Gate"). Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 109
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 112

Revision as of 11:26, 7 October 2016 (view source) Registry (Talk \| contribs)		Latest revision as of 22:32, 19 October 2016 (view source) Lejian (Talk \| contribs)
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	<partinfo>BBa_K1886013 short</partinfo>		<partinfo>BBa_K1886013 short</partinfo>

−	The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations(T7ptag) . The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CL protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CL protein(for more information, see the part"PompC-NOT Gate"). Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.	+	The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by DPD, plsrA promoter activates the expression of T7 RNA polymerase which contains two amber mutations(T7ptag) . The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CI protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CI protein(for more information, see the part"PompC-NOT Gate"). Therefore, when DPD and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.