Difference between revisions of "Part:BBa K2130002"
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This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab. | This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab. | ||
| + | |||
| + | Mutation sites: | ||
| + | Y812H and Y1001H | ||
| + | |||
| + | Team Macquarie helped us made an in-vitro assay for the comparison of WT and mutant 459. | ||
| + | https://static.igem.org/mediawiki/2016/f/f8/T--Macquarie_Australia--CollabSing_.png | ||
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Latest revision as of 22:26, 19 October 2016
Cas9 mutant 459
Cas9 mutant 459
This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.
Mutation sites: Y812H and Y1001H
Team Macquarie helped us made an in-vitro assay for the comparison of WT and mutant 459.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3643
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1188
Illegal BglII site found at 1647
Illegal BglII site found at 1736
Illegal BglII site found at 2381
Illegal BamHI site found at 4105 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]