Difference between revisions of "Part:BBa K2117009"

 
 
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<partinfo>BBa_K2117009 short</partinfo>
 
<partinfo>BBa_K2117009 short</partinfo>
  
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This part is a semi-synthetic shuttle vector plasmid backbone, pSB1A8YL, which is a low copy plasmid that replicates in both E. coli and Yarrowia lipolytica. It encodes an insertion cassette consisting of the prefix10 and suffix10 sequences, ampicillin resistance for selection in E. coli and an URA3 marker for counter selection in Y. lipolytica.
  
 
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Latest revision as of 22:08, 19 October 2016


Plasmid enabling Yarrowia lipolytica biobrick

This part is a semi-synthetic shuttle vector plasmid backbone, pSB1A8YL, which is a low copy plasmid that replicates in both E. coli and Yarrowia lipolytica. It encodes an insertion cassette consisting of the prefix10 and suffix10 sequences, ampicillin resistance for selection in E. coli and an URA3 marker for counter selection in Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4013
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4019
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4013
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4013
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4013
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4028
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2960