Difference between revisions of "Part:BBa K530001"

Revision as of 22:00, 19 October 2016

CRTE

This part has been improved. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2117012">BBa_K2117009</a>

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 335
Illegal XhoI site found at 34
Illegal XhoI site found at 166
Illegal XhoI site found at 985
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 202
1000
COMPATIBLE WITH RFC[1000]

Characterization of performance

Below we can see the beta-carotene production levels on a per cell basis for different combinations of parts involved in beta-carotene synthesis. These are compared with a wild type control. tHMG1 is used to funnel more initial substrate into the pathway and although useful, was not submitted as a part. 2I indicates that two copies of crtI were used.

Plasmid Map

Sequencing

This is the sequencing for colony 4.

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 __NOTOC__
 <partinfo>BBa_K530001 short</partinfo>
+This part has been improved. Please see <a href="https://parts.igem.org/cgi/partsdb/jumper.cgi?search=BBa_K2117012">BBa_K2117009</a>
 Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.