Difference between revisions of "Part:BBa K2150017"

Revision as of 21:56, 19 October 2016

pT7 TetX-GFP(fusion protein)

T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator.

This part is designed to produce a high level of the degrading enzyme in the presence of T7 RNA polymerase(see BBa_K21500028). Sequence and Features

Assembly Compatibility:

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 T7 promoter with RBS, and the fusion protein of tetX and GFP(referred to as tetX-GFP BBa_K2150013) with double terminator.
-The fluorescence intensity of tetX-GFP is lower than that of a single GFP under same circumstances(same promoter, same growth stage, etc.). Besides, though it has been reported that T7 promoter doesn't work with E.coli RNA polymerase, but only with T7 RNA polymerase, we observed green fluorescence in the absence of T7 RNA polymerase. In the presence of T7 RNA polymerase, however, a considerable increase of fluorescence intensity was detected.
-<!-- Add more about the biology of this part here
-===Usage and Biology===
+===Usage and Biology===
+This part is designed to produce a high level of the degrading enzyme in the presence of T7 RNA polymerase(see [https://parts.igem.org/Part:BBa_K2150028 BBa_K21500028]).
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 <span class='h3bb'>Sequence and Features</span>