Difference between revisions of "Part:BBa K105001"
(→‎Improvement)
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===Improvement===
 
===Improvement===
 
2016 SZU-China has improved this parts and give it a new name  
 
2016 SZU-China has improved this parts and give it a new name  
−
<a href="https://parts.igem.org/Part:BBa_K1884002">BBa_K1884002</a>
+
<a href="https://parts.igem.org/Part:BBa_K1884002">(BBa_K1884002)</a>
  
 
<br\n>
 
<br\n>

Revision as of 21:52, 19 October 2016

VP16 - eucaryotic activation domain

Originally VP16 is a protein from the herpes simplex virus. It activates the transcription of the late genes of this virus by the RNA-polymerase II of the eucaryotic host.

In artificial systems VP16 is used in fusion with a DNA-binding domain. This DNA-binding domain is specific for a bait sequence. So the selective expression of genes with a promoter containing this bait sequence can be achieved.

Improvement

2016 SZU-China has improved this parts and give it a new name <a href="https://parts.igem.org/Part:BBa_K1884002">(BBa_K1884002)</a>

<br\n> Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]