Difference between revisions of "Part:BBa K1913024"
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<partinfo>BBa_K1913024 short</partinfo> | <partinfo>BBa_K1913024 short</partinfo> | ||
| − | Synthetic ptet-FixK2 hybrid promoter is | + | Synthetic ptet-FixK2 hybrid promoter is a promoter wich can not only be activated by light sensors YF1-FixJ composite but also inhibited by the TetR repressor. The sequence of the upper control element is from wild type FixK2 promoter on the genomic sequence of <i>Bradyrhizobium japonicum</i>, which contains two typical FixJ boxes. The core element region is replaced by the core element region of BBa_J23106. Additional tet operators are inserted into the core element region of promoter as second control elements which result in transcription repression. |
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Revision as of 21:50, 19 October 2016
Synthetic ptet-FixK2 hybrid promoter with RBS
Synthetic ptet-FixK2 hybrid promoter is a promoter wich can not only be activated by light sensors YF1-FixJ composite but also inhibited by the TetR repressor. The sequence of the upper control element is from wild type FixK2 promoter on the genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. The core element region is replaced by the core element region of BBa_J23106. Additional tet operators are inserted into the core element region of promoter as second control elements which result in transcription repression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 118
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 27
- 1000COMPATIBLE WITH RFC[1000]