Difference between revisions of "Part:BBa K2117004"
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<partinfo>BBa_K2117004 short</partinfo>
 
<partinfo>BBa_K2117004 short</partinfo>
  
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This is a composite part consisting of the constitutive Y. lipolytica TEF1 promoter (BBa_K2117000) and a Y. lipolytica codon-optimized hrGFP (BBa_K2117003).
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This is a promoter optimized for expression of sgRNA in Yarrowia Lipolytica.  
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sgRNA is one of the components needed for a functional CRISPR-Cas9 system. The sgRNA guides the protopacer to the gene of interest inducing a double stranded break.  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 21:45, 19 October 2016


SCR1'-tRNA

This is a promoter optimized for expression of sgRNA in Yarrowia Lipolytica.

sgRNA is one of the components needed for a functional CRISPR-Cas9 system. The sgRNA guides the protopacer to the gene of interest inducing a double stranded break.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]