Difference between revisions of "Part:BBa K1913023"

Revision as of 21:44, 19 October 2016

Synthetic plac-FixK2 hybrid promoter with RBS

Synthetic plac-FixK2 hybrid promoter is a promoter which can not only be activated by light sensors YF1-FixJ but also inhibited by LacI repressor. The sequence of the upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. The core element region is replaced by core element region of ompC promoter from another two-component system, EnvZ-OmpR in Escherichia coli. Additional lac operators on the both side of promoter as second control elements that result in transcription repression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 48
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1913023 short</partinfo>
-Synthetic plac-FixK2 hybrid promoter is the promoter that could not only be activated by light sensors YF1-FixJ composite and but inhibited by LacI repressor. The sequence of upper control element is from wild type FixK2 hybrid promoter on genomic sequence of Bradyrhizobium japonicum, which contains two typical FixJ boxes. And core element region is replaced by core element region of ompC promoter from another two-component systems  EnvZ-OmpR in E. coli. Addition lac operators on the both side of promoter as second control element that results in transcription repression.
+Synthetic plac-FixK2 hybrid promoter is a promoter which can not only be activated by light sensors YF1-FixJ but also inhibited by LacI repressor. The sequence of the upper control element is from wild type FixK2 hybrid promoter on genomic sequence of <i>Bradyrhizobium japonicum</i>, which contains two typical FixJ boxes. The core element region is replaced by core element region of ompC promoter from another two-component system,  EnvZ-OmpR in <i>Escherichia coli</i>. Additional lac operators on the both side of promoter as second control elements that result in transcription repression.
 <!-- Add more about the biology of this part here