Difference between revisions of "Part:BBa K1913020"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K1913020 short</partinfo>
 
<partinfo>BBa_K1913020 short</partinfo>
  
−
mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.
+
mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which makes the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:35, 19 October 2016


mRFP with degradation tag

mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which makes the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]