Difference between revisions of "Part:BBa K1985014"

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<partinfo>BBa_K1985014 short</partinfo>
 
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This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used.  
 
This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used.  
 
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Revision as of 21:25, 19 October 2016

Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

This part is an improved version of the fusion protein(BBa_K1739003) designed by the Kent 2015 iGEM team. It contains four segments, an arabinose inducible promoter designed by the Imperial 2014 iGEM team (Part:BBa_K1321333), the CsgA signal sequence, the first 61 aminoacids of the prion domain Sup35 and the electron transfer protein cytochrome 562. Our improved BioBrick aims to optimize the self-assembly process of amyloid fibrils with the addition of these residues as they have been considered to be a suitable building block for the assembly of functional nanostructures***. The addition of the arabinose inducible promoter allows for tighter control for the expression of amyloid fibres rather than a constitutive promoter as was previously used. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961