Difference between revisions of "Part:BBa K1895000:Experience"
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<p>The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.</p> | <p>The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.</p> | ||
<p>The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.</p> | <p>The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.</p> |
Revision as of 20:53, 19 October 2016
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how you used this part and how it worked out.
Applications of BBa_K1895000
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The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.
The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.
The cells were left to grow for 18 hours with OD and fluorescence being measured every twenty minutes, and orbital shaking occuring between these measurement points. Our results can bee seen below in Figure 1.