Difference between revisions of "Part:BBa K1896014"
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This part constitutively expresses a fusion protein comprising the monomeric GFP variant [[Part:BBa_K1896001|mGFPuv2]] and [[Part:BBa_K1896004|Streptavidin]]. When expressed in ''E. coli'', less fluorescence is observed compared to [[Part:BBa_K1896010|BBa_K1896010]]. Growth is partially inhibited as well, probably due to the tetrameric nature of the Streptavidin domain, which may cause aggregation of the fusion protein. [[Part:BBa_K1896015|BBa_K1896015]] contains a monomeric variant of Streptavidin and performs better. | This part constitutively expresses a fusion protein comprising the monomeric GFP variant [[Part:BBa_K1896001|mGFPuv2]] and [[Part:BBa_K1896004|Streptavidin]]. When expressed in ''E. coli'', less fluorescence is observed compared to [[Part:BBa_K1896010|BBa_K1896010]]. Growth is partially inhibited as well, probably due to the tetrameric nature of the Streptavidin domain, which may cause aggregation of the fusion protein. [[Part:BBa_K1896015|BBa_K1896015]] contains a monomeric variant of Streptavidin and performs better. | ||
| − | + | [[File:BBa K1896001 fusionProteins.jpg|400px|thumb|centre|MGFPuv2 can be used in difficult fusion proteins, from left to right: non-fluorescent control, INP<sub>RC</sub>-mGFPuv2 (weak promoter), mGFPuv2-streptavidin, mGFPuv2-mSA2, mGFPuv2]] | |
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Latest revision as of 20:53, 19 October 2016
mGFPuv2-streptavidin generator
This part constitutively expresses a fusion protein comprising the monomeric GFP variant mGFPuv2 and Streptavidin. When expressed in E. coli, less fluorescence is observed compared to BBa_K1896010. Growth is partially inhibited as well, probably due to the tetrameric nature of the Streptavidin domain, which may cause aggregation of the fusion protein. BBa_K1896015 contains a monomeric variant of Streptavidin and performs better.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 872
Illegal AgeI site found at 923 - 1000COMPATIBLE WITH RFC[1000]