Difference between revisions of "Part:BBa K1895000:Experience"
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<I>iGEM_Newcastle 2016</I>
 
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<p>The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.</p>
 
<p>The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.</p>
 
<p>The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.</p>
 
<p>The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.</p>
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Revision as of 20:47, 19 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1895000

User Reviews

UNIQdf3ac3632c640303-partinfo-00000000-QINU UNIQdf3ac3632c640303-partinfo-00000001-QINU