Difference between revisions of "Part:BBa K1895000:Experience"

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<p>The cells were left to grow for 18 hours with OD and fluorescence being measured every twenty minutes, and orbital shaking occuring between these measurement points. Our results can bee seen below in Figure 1. </p>
 
<p>The cells were left to grow for 18 hours with OD and fluorescence being measured every twenty minutes, and orbital shaking occuring between these measurement points. Our results can bee seen below in Figure 1. </p>
  
<figure><IMG SRC="https://static.igem.org/mediawiki/2016/e/e2/Lightbulb_all_errorbars.PNG" ALT="some text" WIDTH=958 HEIGHT=500>
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<figure><IMG SRC="https://static.igem.org/mediawiki/2016/e/e2/Lightbulb_all_errorbars.PNG" ALT="some text" WIDTH=800HEIGHT=500>
 
<p><figcaption>Figure 8. Summary of the data of all plate reader experiments performed with the modified protocol. It can be seen that temperature does indeed affect the activity of both the P<em><sub>htpG</sub></em> and P<em><sub>dnaK</sub></em> promoters, thus leading to increasing levels of sfGFP expression. We have annotated the three states ('OFF', 'LOW' and 'HIGH') at which our 'lightbulb' devices exist according to our three temperature conditions tested.</figcaption></figure></p>
 
<p><figcaption>Figure 8. Summary of the data of all plate reader experiments performed with the modified protocol. It can be seen that temperature does indeed affect the activity of both the P<em><sub>htpG</sub></em> and P<em><sub>dnaK</sub></em> promoters, thus leading to increasing levels of sfGFP expression. We have annotated the three states ('OFF', 'LOW' and 'HIGH') at which our 'lightbulb' devices exist according to our three temperature conditions tested.</figcaption></figure></p>
  
 
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Revision as of 20:40, 19 October 2016


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Please enter how you used this part and how it worked out.

Applications of BBa_K1895000

User Reviews

UNIQ1f5e6a086f1e1ce8-partinfo-00000000-QINU UNIQ1f5e6a086f1e1ce8-partinfo-00000001-QINU

The cells containing the BBa_K1895000 device were grown up in liquid culture of LB broth (with chloramphenicol in strains containing a pSB1C3 housed construct) overnight at 37°C. The following day, the bacterial cells were diluted down to an appropriate optical density in the region of 0.05 at 600 nm using LB broth with 0.034mg/ml chloramphenicol.

The plate reader was then set at either 30°C, 37°C and 42 °C and measured for growth using OD600 and fluorescence of GFP using 485 nm excitation wavelength and 520 nm emission wavelength with a gain of 1000.

The cells were left to grow for 18 hours with OD and fluorescence being measured every twenty minutes, and orbital shaking occuring between these measurement points. Our results can bee seen below in Figure 1.

some text

Figure 8. Summary of the data of all plate reader experiments performed with the modified protocol. It can be seen that temperature does indeed affect the activity of both the PhtpG and PdnaK promoters, thus leading to increasing levels of sfGFP expression. We have annotated the three states ('OFF', 'LOW' and 'HIGH') at which our 'lightbulb' devices exist according to our three temperature conditions tested.

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