Difference between revisions of "Part:BBa K1992001:Design"
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==design notes== | ==design notes== | ||
The changes in the sequence were 4 nucleotides replacement:<br> | The changes in the sequence were 4 nucleotides replacement:<br> | ||
− | 'g' | + | 'g' to 'c' - 99 bp<br> |
− | 'g' | + | 'g' to 'a' - 402 bp<br> |
− | 'g' | + | 'g' to 'a' - 405 bp<br> |
− | 'a' | + | 'a' to 'g' - 996 bp<br> |
<!-- ######################## SUB TITLE ######################### --> | <!-- ######################## SUB TITLE ######################### --> |
Latest revision as of 20:37, 19 October 2016
PctA-Tar hybrid receptor
design notes
The changes in the sequence were 4 nucleotides replacement:
'g' to 'c' - 99 bp
'g' to 'a' - 402 bp
'g' to 'a' - 405 bp
'a' to 'g' - 996 bp
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 270
Illegal NgoMIV site found at 351
Illegal NgoMIV site found at 355
Illegal NgoMIV site found at 374
Illegal AgeI site found at 474
Illegal AgeI site found at 685
Illegal AgeI site found at 1540 - 1000COMPATIBLE WITH RFC[1000]