Difference between revisions of "Part:BBa K2150014"
Forevyoung (Talk | contribs) |
Forevyoung (Talk | contribs) |
||
| Line 13: | Line 13: | ||
In the presence of tetR, this part can be induced by the addition of atc(Fig.1). | In the presence of tetR, this part can be induced by the addition of atc(Fig.1). | ||
| − | [[File: Simulator_1.png|250px]] | + | [[File:Simulator_1.png|250px]] |
<!-- --> | <!-- --> | ||
Revision as of 20:35, 19 October 2016
pTet GFP
This part is composed of pTet promoter, a high efficiency RBS, coding region of GFP and double terminator. Sinve pTet promoter is constitutively on and can be repressed by TetR, this part is used to test whether pTet is sensitive to TetR.
Usage and Biology
This part was used in our project to form a simulation system with BBa_K2150022 to test our designs. This part produced a higher level of GFP than BBa_K2150022(see here), when the concentration of aTc is 1ug/mL.
Characterization
In the presence of tetR, this part can be induced by the addition of atc(Fig.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724