Difference between revisions of "Part:BBa K2150015"
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<partinfo>BBa_K2150015 short</partinfo>
 
<partinfo>BBa_K2150015 short</partinfo>
  
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This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR, and the repression can be inhibited by tetracycline and its analogs. This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli.  
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This part is tetX-GFP with pTet promoter.  
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part is compared with BBa_K2150027. The data showes BBa_K2150027 has a better capacity of degrading tetracycline than this part(see [https://parts.igem.org/Part:BBa_K2150027 BBa_K21500027]).
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===Characterization===
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This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see [https://parts.igem.org/Part:BBa_K2150013 BBa_K2150013]).
  
 
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Revision as of 20:34, 19 October 2016


pTet TetX-GFP(fusion protein)

This part is tetX-GFP with pTet promoter.

Usage and Biology

We use this part to create an antibiotic scavenger in the presence of tetR. The performance of this part is compared with BBa_K2150027. The data showes BBa_K2150027 has a better capacity of degrading tetracycline than this part(see BBa_K21500027).

Characterization

This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli (see BBa_K2150013).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939