Difference between revisions of "Part:BBa K1896020"
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| − | + | This part can be used to construct cloning vectors that are compatible with Golden Gate assembly.[1] | |
| − | + | Two diverging BsaI cloning sites are situated inbetween a weak to medium constitutive promoter+RBS and double terminator. | |
| + | One or more linear fragments can be cloned into this vector in an efficient one-pot restriction and ligation reaction. | ||
| + | The outer sticky ends are GATG and TACT and the resulting constructs are indistinguishable from those created by | ||
| + | standard Registry assembly methods, since the same scars are generated. | ||
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<partinfo>BBa_K1896020 parameters</partinfo> | <partinfo>BBa_K1896020 parameters</partinfo> | ||
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| + | ===References=== | ||
| + | # Engler, C., Gruetzner, R., Kandzia, R., & Marillonnet, S. (2009). Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. ''PloS one'', 4(5), e5553. | ||
Revision as of 20:19, 19 October 2016
pXW-GG
This part can be used to construct cloning vectors that are compatible with Golden Gate assembly.[1]
Two diverging BsaI cloning sites are situated inbetween a weak to medium constitutive promoter+RBS and double terminator.
One or more linear fragments can be cloned into this vector in an efficient one-pot restriction and ligation reaction.
The outer sticky ends are GATG and TACT and the resulting constructs are indistinguishable from those created by
standard Registry assembly methods, since the same scars are generated.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 77
Illegal BsaI.rc site found at 66
References
- Engler, C., Gruetzner, R., Kandzia, R., & Marillonnet, S. (2009). Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. PloS one, 4(5), e5553.