Difference between revisions of "Part:BBa K1896011"

Revision as of 19:46, 19 October 2016

inaZ (INP) generator

This is an improved version of BBa_K584027, which expresses an Ice Nucleating Protein, encoded by the inaZ gene of Pseudomonas syringae. The original part was not genetically stable, probably due to toxicity caused by overexpression of the membrane bound INP. Replacing the promoter with a weaker variant proved to be effective in reducing toxicity, while still maintaining activity as biological ice nucleator. This activity is the result of a central repeat domain that catalyses the formation of ice crystals by binding molecules of water in a way that resembles the lattice structure of ice. [1]

Addition of E. coli expressing mRFP to supercooled water has no effect, addition of a culture containing this part instantly causes ice nucleation catalysed by INP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 707
Illegal NgoMIV site found at 2219
Illegal NgoMIV site found at 2363
Illegal NgoMIV site found at 2627
Illegal NgoMIV site found at 2771
Illegal NgoMIV site found at 2819
Illegal NgoMIV site found at 2939
Illegal AgeI site found at 478
Illegal AgeI site found at 1883
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 299

References

Pandey, R., Usui, K., Livingstone, R. A., Fischer, S. A., Pfaendtner, J., Backus, E. H., ... & Scheel, J. F. (2016). Ice-nucleating bacteria control the order and dynamics of interfacial water. Science advances, 2(4), e1501630.

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+Addition of ''E. coli'' expressing mRFP to supercooled water has no effect,  addition of a culture containing this part instantly causes ice nucleation catalysed by INP.